Study with Quizlet and memorize flashcards containing terms like to complete the concept map below, categorize DNA tools as either those that detect specific genes within DNA or those that change an individual's DNA. not all labels will be used., classify each of the following as DNA that is "translated into proteins" and DNA that is "not translated into proteins.", put the labels in the
2. First-generation DNA sequencing. Watson and Crick famously solved the three-dimensional structure of DNA in 1953, working from crystallographic data produced by Rosalind Franklin and Maurice Wilkins [2], [3], which contributed to a conceptual framework for both DNA replication and encoding proteins in nucleic acids.
Mitochondrial DNA is only a small portion of the DNA in a eukaryotic cell; most of the DNA can be found in the cell nucleus and, in plants and algae, also in plastids such as chloroplasts. Human mitochondrial DNA was the first significant part of the human genome to be sequenced. [4]
16S rRNA sequencing. Shotgun Metagenomic Sequencing. Cost ~$50 USD. Starting at ~$150 but price will depend on sequencing depth required. Sample preparation. Similar complexity to shotgun sequencing. Similar complexity to 16S rRNA sequencing. Functional profiling (profile microbial genes) No (but ‘predicted’ functional profiling is possible)
Bisulfite conversion sequencing can be done with targeted methods such as amplicon methyl-seq or target enrichment, or with whole-genome bisulfite sequencing. Additionally, alternative chemistries like OxBS and TAB-Seq can be used with NGS for identification of hydroxymethylation (5-hMc) in conjunction with methylation (5-mc) analysis. See the
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dna sequencing vs dna profiling